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Article

Structural and kinetic studies on the Ser101Ala variant of choline oxidase: catalysis by compromise.

Archives of biochemistry and biophysics - 15 Sept 2010

Finnegan Steffan, Yuan Hongling, Wang Yuan-Fang, Orville Allen M, Weber Irene T, Gadda Giovanni

Abstract excerpt

The oxidation of choline catalyzed by choline oxidase includes two reductive half-reactions where FAD is reduced by the alcohol substrate and by an aldehyde intermediate transiently formed in the reaction. Each reductive half-reaction is followed by an oxidative half-reaction where the reduced flavin is oxidized by oxygen. Here, we have used mutagenesis to prepare the Ser101Ala mutant of choline oxidase and have...

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Identifiers

PMID: 20561507
DOI: 10.1016/j.abb.2010.06.014

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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