Article
Rescuing of the hydride transfer reaction in the Glu312Asp variant of choline oxidase by a substrate analogue.
Archives of biochemistry and biophysics - 1 Jul 2010
Quaye Osbourne, Nguyen Tranbao, Gannavaram Swathi, Pennati Andrea, Gadda Giovanni
Abstract excerpt
In the active site of choline oxidase, Glu312 participates in binding the trimethylammonium group of choline, thereby positioning the alcohol substrate properly for efficient hydride transfer to the enzyme-bound flavin. Previous studies have shown that substitution of Glu312 with aspartate results in a perturbed mechanism of hydride transfer, with a 260-fold decrease in the rate associated with the mutation....
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