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Article

Evidence for proton tunneling and a transient covalent flavin-substrate adduct in choline oxidase S101A.

Biochimica et biophysica acta. Proteins and proteomics - 1 Nov 2017

Uluisik Rizvan, Romero Elvira, Gadda Giovanni

Abstract excerpt

The effect of temperature on the reaction of alcohol oxidation catalyzed by choline oxidase was investigated with the S101A variant of choline oxidase. Anaerobic enzyme reduction in a stopped-flow spectrophotometer was biphasic using either choline or 1,2-[2H4]-choline as a substrate. The limiting rate constants klim1 and klim2 at saturating substrate were well separated (klim1/klim2>9), and were >15-fold slower...

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Source

Identifiers

PMID: 28843728
DOI: 10.1016/j.bbapap.2017.08.004

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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