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Article

Systematic analysis of essential genes reveals important regulators of G protein signaling.

Molecular cell - 11 Jun 2010

Cappell Steven D, Baker Rachael, Skowyra Dorota, Dohlman Henrik G

Abstract excerpt

The yeast pheromone pathway consists of a canonical heterotrimeric G protein and MAP kinase cascade. To identify additional signaling components, we systematically evaluated 870 essential genes using a library of repressible-promoter strains. Quantitative transcription-reporter and MAPK activity assays were used to identify strains that exhibit altered pheromone sensitivity. Of the 92 newly identified essential...

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Identifiers

PMID: 20542006
DOI: 10.1016/j.molcel.2010.05.026
PMCID: PMC2919228

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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