Article
Epitope mapping in cell surface proteins by site-directed masking: defining the structural elements of NTPDase3 inhibition by a monoclonal antibody.
Protein engineering, design & selection : PEDS - 1 Jul 2010
Ivanenkov Vasily V, Crawford Patrick A, Toyama Aimi, Sévigny Jean, Kirley Terence L
Abstract excerpt
We adapted the method of epitope mapping by site-directed masking, which was described for purified soluble antigens [Paus,D. and Winter,G. (2006) Proc. Natl Acad. Sci. USA, 103, 9172-9177.], to map the binding site of an inhibitory monoclonal antibody on the cell surface protein ecto-nucleotidase NTPDase3. Using homology modeling, we built a 3D structure of NTPDase3 and designed 21 single cysteine mutations...
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