Article
Mapping the paratope of anti-CD4 recombinant Fab 13B8.2 by combining parallel peptide synthesis and site-directed mutagenesis.
The Journal of biological chemistry - 18 Apr 2003
Bès Cédric, Briant-Longuet Laurence, Cerutti Martine, Heitz Frédéric, Troadec Samuel, Pugnière Martine, Roquet Francoise, Molina Franck, Casset Florence, Bresson Damien, Péraldi-Roux Sylvie, Devauchelle Gérard, Devaux Christian, Granier Claude, Chardes Thierry
Abstract excerpt
We analyzed antigen-binding residues from the variable domains of anti-CD4 antibody 13B8.2 using the Spot method of parallel peptide synthesis. Sixteen amino acids, defined as Spot critical residues (SCR), were identified on the basis of a 50% decrease in CD4 binding to alanine analogs of reactive peptides. Recombinant Fab 13B8.2 mutants were constructed with alanine residues in place of each of the 16 SCR,...
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