Article
Fine-structure analysis of the P1 plasmid partition site.
Journal of bacteriology - 1 Jun 1991
Martin K A, Davis M A, Austin S
Abstract excerpt
P1 plasmid partition requires two plasmid-encoded Par proteins and a cis-acting site. The site, parS, lies in a region consisting of a 13-bp palindrome and an adjacent AT-rich sequence. A series of point mutations were analyzed for their effects on partition site activity. The results indicated that only the left arm of the palindrome and some adjacent bases were needed. The limits of the functional site were...
Topics
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Escherichia coli
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids