Article
A single parS sequence from the cluster of four sites closest to oriC is necessary and sufficient for proper chromosome segregation in Pseudomonas aeruginosa.
PloS one - 1 Jan 2015
Jecz Paulina, Bartosik Aneta A, Glabski Krzysztof, Jagura-Burdzy Grazyna
Abstract excerpt
Among the mechanisms that control chromosome segregation in bacteria are highly-conserved partitioning systems comprising three components: ParA protein (a deviant Walker-type ATPase), ParB protein (a DNA-binding element) and multiple cis-acting palindromic centromere-like sequences, designated parS. Ten putative parS sites have been identified in the P. aeruginosa PAO1 genome, four localized in close proximity...
Topics
- Alleles
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Chromosome Segregation
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA, Bacterial
- Gene Silencing
- Mutation
- Nucleotide Motifs