Article
Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations.
Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990
Schild D, Brake A J, Kiefer M C, Young D, Barr P J
Abstract excerpt
Functional complementation of mutations in the yeast Saccharomyces cerevisiae has been used to clone three multifunctional human genes involved in de novo purine biosynthesis. A HepG2 cDNA library constructed in a yeast expression vector was used to transform yeast strains with mutations in adeni...
Topics
- Acyltransferases
- Adenine
- Amino Acid Sequence
- Base Sequence
- Carbon-Nitrogen Ligases
- Carboxy-Lyases
- Cloning, Molecular
- Gene Library
- Genes
- Genetic Complementation Test
- Humans
- Hydroxymethyl and Formyl Transferases
- Ligases
- Molecular Sequence Data
- Multienzyme Complexes