Article
Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations.
Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990
Schild D, Brake A J, Kiefer M C, Young D, Barr P J
Abstract excerpt
Functional complementation of mutations in the yeast Saccharomyces cerevisiae has been used to clone three multifunctional human genes involved in de novo purine biosynthesis. A HepG2 cDNA library constructed in a yeast expression vector was used to transform yeast strains with mutations in adenine biosynthetic genes. Clones were isolated that complement mutations in the yeast ADE2, ADE3, and ADE8 genes. The cDNA...
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