Article
Expression of a human cDNA encoding a protein containing GAR synthetase, AIR synthetase, and GAR transformylase corrects the defects in mutant Chinese hamster ovary cells lacking these activities.
Somatic cell and molecular genetics - 1 Jul 1991
Chang F H, Barnes T S, Schild D, Gnirke A, Bleskan J, Patterson D
Abstract excerpt
The isolation of a human cDNA encoding the multifunctional protein containing GAR synthetase, AIR synthetase, and GAR transformylase by functional complementation of purine auxotrophy in yeast has been reported. Chinese hamster ovary (CHO) cell mutant purine auxotrophs deficient in GAR synthetase (Ade-C) or AIR synthetase plus GAR transformylase (Ade-G) activities were transfected with this human GART cDNA...
Topics
- Acyltransferases
- Animals
- Carbon-Nitrogen Ligases
- Cell Line
- Cricetinae
- Cricetulus
- DNA
- Female
- Gene Expression
- Genes
- Humans