Article
Quantum mechanical hydrogen tunneling in bacterial copper amine oxidase reaction.
Biochemical and biophysical research communications - 7 Apr 2006
Murakawa Takeshi, Okajima Toshihide, Kuroda Shun'ichi, Nakamoto Takuya, Taki Masayasu, Yamamoto Yukio, Hayashi Hideyuki, Tanizawa Katsuyuki
Abstract excerpt
A key step decisively affecting the catalytic efficiency of copper amine oxidase is stereospecific abstraction of substrate alpha-proton by a conserved Asp residue. We analyzed this step by pre-steady-state kinetics using a bacterial enzyme and stereospecifically deuterium-labeled substrates, 2-phenylethylamine and tyramine. A small and temperature-dependent kinetic isotope effect (KIE) was observed with...
Topics
- Amine Oxidase (Copper-Containing)
- Amino Acid Substitution
- Bacterial Proteins
- Crystallography, X-Ray
- Deuterium
- Dihydroxyphenylalanine
- Kinetics
- Mutation
- Oxidation-Reduction
- Phenethylamines