Article
A modified "cross-talk" between histone H2B Lys-120 ubiquitination and H3 Lys-79 methylation.
The Journal of biological chemistry - 9 Jul 2010
Darwanto Agus, Curtis Matthew P, Schrag Matthew, Kirsch Wolff, Liu Peng, Xu Guoliang, Neidigh Jonathan W, Zhang Kangling
Abstract excerpt
Western blot analysis is currently the major method utilized for quantitatively assessing histone global modifications. However, there is a growing need to develop a highly specific, accurate, and multisite quantitative method. Herein, we report a liquid chromatography-tandem mass spectrometry-multiple reaction monitoring method to simultaneously quantify multisite modifications with unmatched specificity,...
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