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Article

Optimized variants of the cold shock protein from in vitro selection: structural basis of their high thermostability.

Journal of molecular biology - 15 Jun 2007

Max Klaas E A, Wunderlich Michael, Roske Yvette, Schmid Franz X, Heinemann Udo

Abstract excerpt

The bacterial cold shock proteins (Csp) are widely used as models for the experimental and computational analysis of protein stability. In a previous study, in vitro evolution was employed to identify strongly stabilizing mutations in Bs-CspB from Bacillus subtilis. The best variant found by this approach contained the mutations M1R, E3K and K65I, which raised the midpoint of thermal unfolding of Bs-CspB from...

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Identifiers

PMID: 17481655
DOI: 10.1016/j.jmb.2007.04.016

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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