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Article

Engineering stability into Escherichia coli secreted Fabs leads to increased functional expression.

Protein engineering, design & selection : PEDS - 1 Jul 2006

Demarest Stephen J, Chen Gang, Kimmel Bruce E, Gustafson David, Wu Jane, Salbato Jared, Poland John, Elia Marikka, Tan Xuqiu, Wong Ken, Short Jay, Hansen Geneviève

Abstract excerpt

The recombinant expression of immunoglobulin domains, Fabs and scFvs in particular, in Escherichia coli can vary significantly from antibody to antibody. We hypothesized that poor Fab expression is often linked to poor intrinsic stability. To investigate this further, we applied a novel approach for stabilizing a poorly expressing anti-tetanus toxoid human Fab with a predisposition for being misfolded and...

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Source

Identifiers

PMID: 16672248
DOI: 10.1093/protein/gzl016

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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