Article
Frameshift mutagenesis and microsatellite instability induced by human alkyladenine DNA glycosylase.
Molecular cell - 26 Mar 2010
Klapacz Joanna, Lingaraju Gondichatnahalli M, Guo Haiwei H, Shah Dharini, Moar-Shoshani Ayelet, Loeb Lawrence A, Samson Leona D
Abstract excerpt
Human alkyladenine DNA glycosylase (hAAG) excises alkylated purines, hypoxanthine, and etheno bases from DNA to form abasic (AP) sites. Surprisingly, elevated expression of hAAG increases spontaneous frameshift mutagenesis. By random mutagenesis of eight active site residues, we isolated hAAG-Y127I/H136L double mutant that induces even higher rates of frameshift mutation than does the wild-type hAAG; the Y127I...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.