Article
Synthesis of large rRNAs by RNA polymerase II in mutants of Saccharomyces cerevisiae defective in RNA polymerase I.
Proceedings of the National Academy of Sciences of the United States of America - 1 May 1991
Nogi Y, Yano R, Nomura M
Abstract excerpt
The 35S rRNA gene of the yeast Saccharomyces cerevisiae was fused to the GAL7 promoter. This hybrid gene, when present on a multicopy plasmid and induced by galactose, suppressed the growth defects of a temperature-sensitive RNA polymerase I (pol I) mutant and those of a mutant in which the gene for the second largest subunit of pol I was deleted. Analysis of pulse-labeled RNA directly demonstrated that rRNA...
Topics
- Base Sequence
- Blotting, Southern
- DNA, Fungal
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- RNA Polymerase I
- RNA Polymerase II
- RNA, Fungal
- RNA, Ribosomal
- Saccharomyces cerevisiae