Article
Expression, purification and characterization of leukotriene B(4) receptor, BLT1 in Pichia pastoris.
Protein expression and purification - 1 Jul 2010
Hori Tetsuya, Sato Yo, Takahashi Naoko, Takio Koji, Yokomizo Takehiko, Nakamura Motonao, Shimizu Takao, Miyano Masashi
Abstract excerpt
The high yield expression of BLT1, a G-protein coupled receptor for leukotriene B(4), was established in Pichia pastoris for structural studies. Guinea pig BLT1 was expressed in a functional form without post-translational modifications for the rapid purification and the crystallization. Among th...
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