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Article

Expression, purification and characterization of leukotriene B(4) receptor, BLT1 in Pichia pastoris.

Protein expression and purification - 1 Jul 2010

Hori Tetsuya, Sato Yo, Takahashi Naoko, Takio Koji, Yokomizo Takehiko, Nakamura Motonao, Shimizu Takao, Miyano Masashi

Abstract excerpt

The high yield expression of BLT1, a G-protein coupled receptor for leukotriene B(4), was established in Pichia pastoris for structural studies. Guinea pig BLT1 was expressed in a functional form without post-translational modifications for the rapid purification and the crystallization. Among the BLT1s from four species, only guinea pig BLT1 was successfully expressed with the comparable binding affinity to BLT1...

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Source

Identifiers

PMID: 20188179
DOI: 10.1016/j.pep.2010.02.013

Genes

LTB

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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