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Article

Gene-specific FACS sorting method for target selection in high-throughput amplicon sequencing.

BMC genomics - 26 Feb 2010

Sandberg Julia, Neiman Marten, Ahmadian Afshin, Lundeberg Joakim

Abstract excerpt

BACKGROUND: In addition to shotgun sequencing, next generation sequencing has been shown to be suitable for deep sequencing of many specific PCR-amplified target genes in parallel. However, unspecific product formation is a common problem in amplicon sequencing since these fragments are difficult to fully remove by gel purification, and their presence inevitably reduces the number of mappable sequence reads that...

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PMID: 20184782
DOI: 10.1186/1471-2164-11-140
PMCID: PMC2842249

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