Article
Gene-specific FACS sorting method for target selection in high-throughput amplicon sequencing.
BMC genomics - 26 Feb 2010
Sandberg Julia, Neiman Marten, Ahmadian Afshin, Lundeberg Joakim
Abstract excerpt
BACKGROUND: In addition to shotgun sequencing, next generation sequencing has been shown to be suitable for deep sequencing of many specific PCR-amplified target genes in parallel. However, unspecific product formation is a common problem in amplicon sequencing since these fragments are difficult...
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