Targeted Amplicon Genotyping by Sanger Sequencing.

doi:10.1007/978-1-0716-4642-7_14

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Targeted Amplicon Genotyping by Sanger Sequencing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Torres Anthony, Gaudino Reginald

Abstract excerpt

This chapter presents a protocol for Sanger sequencing, a conventional yet rapid, cost-effective, and highly accurate method for targeted sequencing. It involves designing specific primers for forward and reverse template extension reactions, during which fluorophore labeled, chain terminating dideoxynucleotides are incorporated into newly replicated DNA molecules. The resulting end-labeled terminated products...

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DNA
DNA Primers
Genotype
Genotyping Techniques
High-Throughput Nucleotide Sequencing
Polymerase Chain Reaction
Sequence Analysis, DNA

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PMID: 40580294
DOI: 10.1007/978-1-0716-4642-7_14

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

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