Remodeling of the AB site of rat parvalbumin and oncomodulin into a canonical EF-hand.

doi:10.1046/j.1432-1327.1999.00650.x

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Article

Remodeling of the AB site of rat parvalbumin and oncomodulin into a canonical EF-hand.

European journal of biochemistry - 1 Sept 1999

Cox J A, Durussel I, Scott D J, Berchtold M W

Abstract excerpt

Parvalbumin (PV) and the homologous protein oncomodulin (OM) contain three EF-hand motifs, but the first site (AB) cannot bind Ca2+. Here we aimed to recreate the putative ancestral proteins [D19-28E]PV and [D19-28E]OM by replacing the 10-residue-long nonfunctional loop in the AB site by a 12-residue canonical loop. To create an optical conformational probe we also expressed the homologs with a F102W replacement....

Topics

Amino Acid Sequence
Animals
Binding Sites
Calcium
Calcium-Binding Proteins
Circular Dichroism
In Vitro Techniques
Magnesium
Molecular Sequence Data
Mutation
Parvalbumins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Conformation

Protein Structure, Secondary

Rats

Recombinant Proteins

Spectrometry, Fluorescence

Spectrophotometry, Ultraviolet

Source

Open in PubMed

Identifiers

PMID: 10491125
DOI: 10.1046/j.1432-1327.1999.00650.x

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