Article
T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by antagonizing stable DNA strand separation near the transcription start site.
Proceedings of the National Academy of Sciences of the United States of America - 2 Feb 2010
Cámara Beatriz, Liu Minhao, Reynolds Jonathan, Shadrin Andrey, Liu Bing, Kwok King, Simpson Peter, Weinzierl Robert, Severinov Konstantin, Cota Ernesto, Matthews Steve, Wigneshweraraj Siva R
Abstract excerpt
Infection of Escherichia coli by the T7 phage leads to rapid and selective inhibition of the host RNA polymerase (RNAP)--a multi-subunit enzyme responsible for gene transcription--by a small ( approximately 7 kDa) phage-encoded protein called Gp2. Gp2 is also a potent inhibitor of E. coli RNAP in vitro. Here we describe the first atomic resolution structure of Gp2, which reveals a distinct run of surface-exposed...
Topics
- Bacteriophage T7
- Binding Sites
- DNA, Bacterial
- DNA-Directed RNA Polymerases
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Genes, Viral
- Models, Molecular