Article
Identification of bacteriophage N4 virion RNA polymerase-nucleic acid interactions in transcription complexes.
The Journal of biological chemistry - 23 Jan 2009
Davydova Elena K, Kaganman Irene, Kazmierczak Krystyna M, Rothman-Denes Lucia B
Abstract excerpt
Bacteriophage N4 mini-virion RNA polymerase (mini-vRNAP), the 1106-amino acid transcriptionally active domain of vRNAP, recognizes single-stranded DNA template-containing promoters composed of conserved sequences and a 3-base loop-5-base pair stem hairpin structure. The major promoter recognition determinants are a purine located at the center of the hairpin loop (-11G) and a base at the hairpin stem (-8G)....
Topics
- Bacteriophage N4
- Catalytic Domain
- DNA
- DNA-Directed RNA Polymerases
- Genes, Reporter
- Mutation
- Nucleic Acids
- Peptides
- Phosphorylation
- Promoter Regions, Genetic
- Protein Binding
- RNA
- Transcription, Genetic
- Virion