Article
Potassium is critical for the Ni(II)-responsive DNA-binding activity of Escherichia coli NikR.
Journal of the American Chemical Society - 10 Feb 2010
Wang Sheila C, Li Yanjie, Robinson Carol V, Zamble Deborah B
Abstract excerpt
The Escherichia coli NikR transcription factor is a Ni(II)-dependent repressor that regulates the production of a nickel ion transporter. The X-ray crystal structure of the Ni(II)-NikR-DNA bound complex revealed a K(+)-binding site positioned at the interface of the metal- and DNA-binding domains, but the significance of the potassium was unclear. Mutation of one of the K(+) ligands impairs the affinity and...
Topics
- Binding Sites
- Crystallography, X-Ray
- DNA
- Escherichia coli
- Escherichia coli Proteins
- Models, Molecular
- Mutation
- Nickel
- Potassium
- Protein Binding
- Repressor Proteins