Article
Structural and mechanistic insights into Helicobacter pylori NikR activation.
Nucleic acids research - 1 May 2010
Bahlawane C, Dian C, Muller C, Round A, Fauquant C, Schauer K, de Reuse H, Terradot L, Michaud-Soret I
Abstract excerpt
NikR is a transcriptional metalloregulator central in the mandatory response to acidity of Helicobacter pylori that controls the expression of numerous genes by binding to specific promoter regions. NikR/DNA interactions were proposed to rely on protein activation by Ni(II) binding to high-affinity (HA) and possibly secondary external (X) sites. We describe a biochemical characterization of HpNikR mutants that...
Topics
- Bacterial Proteins
- DNA-Binding Proteins
- Models, Molecular
- Mutation
- Nickel
- Protein Binding
- Repressor Proteins
- Scattering, Small Angle
- X-Ray Diffraction