Article
Nonspecific interactions between Escherichia coli NikR and DNA are critical for nickel-activated DNA binding.
Biochemistry - 9 Oct 2012
Krecisz Sandra, Jones Michael D, Zamble Deborah B
Abstract excerpt
The Escherichia coli transcription factor NikR is responsible for nickel-mediated repression of the operon encoding the Nik uptake transporter. The crystal structure of Ni(II)-NikR bound to the nik operator sequence revealed that residues in the loop preceding helix α3 in the metal-binding domain, which becomes structurally ordered upon stoichiometric nickel binding, interact with the DNA backbone. Here, we show...
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