Article
Purification and characterization of the periplasmic nickel-binding protein NikA of Escherichia coli K12.
European journal of biochemistry - 1 Feb 1995
de Pina K, Navarro C, McWalter L, Boxer D H, Price N C, Kelly S M, Mandrand-Berthelot M A, Wu L F
Abstract excerpt
The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system specific for nickel. In this report, we describe the overproduction of the periplasmic nickel-binding protein NikA by cloning the nikA gene into an overexpression vector, pRE1. NikA was purified free of nickel to near homogeneity from the periplasm by hydrophobic and ion-exchange chromatography. N-terminal amino...
Topics
- Bacterial Proteins
- Base Sequence
- Biological Transport, Active
- Chemotaxis
- Cloning, Molecular
- DNA Topoisomerases, Type I
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genetic Vectors