Article
Rare homologous gene targeting in Histoplasma capsulatum: disruption of the URA5Hc gene by allelic replacement.
Journal of bacteriology - 1 Oct 1998
Woods J P, Retallack D M, Heinecke E L, Goldman W E
Abstract excerpt
URA5 genes encode orotidine-5'-monophosphate pyrophosphorylase (OMPpase), an enzyme involved in pyrimidine biosynthesis. We cloned the Histoplasma capsulatum URA5 gene (URA5Hc) by using a probe generated by PCR with inosine-rich primers based on relatively conserved sequences in OMPpases from oth...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Gene Targeting
- Genes, Fungal
- Histoplasma
- Molecular Sequence Data
- Orotate Phosphoribosyltransferase
- Restriction Mapping
- Sequence Analysis, DNA
- Transformation, Genetic