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Article

Rare homologous gene targeting in Histoplasma capsulatum: disruption of the URA5Hc gene by allelic replacement.

Journal of bacteriology - 1 Oct 1998

Woods J P, Retallack D M, Heinecke E L, Goldman W E

Abstract excerpt

URA5 genes encode orotidine-5'-monophosphate pyrophosphorylase (OMPpase), an enzyme involved in pyrimidine biosynthesis. We cloned the Histoplasma capsulatum URA5 gene (URA5Hc) by using a probe generated by PCR with inosine-rich primers based on relatively conserved sequences in OMPpases from other organisms. Transformation with this gene restored uracil prototrophy and OMPpase activity to UV-mutagenized ura5...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 9748447
DOI: 10.1128/JB.180.19.5135-5143.1998
PMCID: PMC107550

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Source receipt: pubmed, retrieved 7 Sept 2026.

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