Article
A mutation in the large subunit ribosomal RNA gene of Tetrahymena confers anisomycin resistance and cold sensitivity.
Genetics - 1 Feb 1991
Sweeney R, Yao C H, Yao M C
Abstract excerpt
Anisomycin, an antibiotic that specifically inhibits the peptidyl transfer function of eukaryotic ribosomes, has been used to select resistant mutants in Tetrahymena thermophila. A mutation conferring anisomycin resistance (an-r) has been localized to a 1.2-kb fragment of the large subunit ribosomal RNA (rRNA) gene by transformation via microinjection. A single base pair change was detected within this region....
Topics
- Animals
- Anisomycin
- Base Sequence
- Cloning, Molecular
- Cold Temperature
- Crosses, Genetic
- Cycloheximide
- DNA, Ribosomal
- Drug Resistance
- Kinetics
- Molecular Sequence Data
- Mutation