Article
Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in Tetrahymena thermophila.
Proceedings of the National Academy of Sciences of the United States of America - 18 Feb 1997
Hai B, Gorovsky M A
Abstract excerpt
The haploid Tetrahymena thermophila genome contains a single alpha-tubulin (ATU) gene. Using biolistic transformation, we disrupted one of the two copies of the ATU gene in the diploid germ-line micronucleus. The heterozygous germ-line transformants were made homozygous in the micronucleus by mat...
Topics
- Animals
- Cell Fusion
- Cell Nucleus
- Crosses, Genetic
- Gene Dosage
- Gene Transfer Techniques
- Genes, Lethal
- Genes, Protozoan
- Genetic Vectors
- Genome, Protozoan
- Heterozygote
- Homozygote
- Micronucleus, Germline
- Mutation
- Ploidies
- Tetrahymena thermophila
- Transformation, Genetic
- Tubulin