Article
An antisense approach to phenotype-based gene cloning in Tetrahymena.
Proceedings of the National Academy of Sciences of the United States of America - 17 Jul 2001
Chilcoat N D, Elde N C, Turkewitz A P
Abstract excerpt
We report a pioneering approach using Tetrahymena thermophila that permits rapid identification of genes based on their null or hypomorphic phenotypes. This technique involves cell transformation with a library of plasmids that encode 26S ribosomal subunits containing short insertions. The insertions correspond to antisense sequences for a large number of genes. The majority of cells each acquires a single...
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