Article
Mutant aminoacyl-tRNA synthetase that compensates for a mutation in the major identity determinant of its tRNA.
Biochemistry - 12 Mar 1991
Miller W T, Hou Y M, Schimmel P
Abstract excerpt
A single G3.U70 base pair in the acceptor helix is the major determinant for the identity of alanine transfer RNAs (Hou & Schimmel, 1988). Introduction of this base pair into foreign tRNA sequences confers alanine acceptance on them. Moreover, small RNA helices with as few as seven base pairs can be aminoacylated with alanine, provided that they encode the critical base pair (Francklyn & Schimmel, 1989)....
Topics
- Amino Acyl-tRNA Synthetases
- Base Sequence
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Mutation
- Phenotype
- Plasmids
- RNA, Transfer, Ala
- Restriction Mapping