Article
Functional compensation of a recognition-defective transfer RNA by a distal base pair substitution.
Biochemistry - 27 Oct 1992
Hou Y M, Schimmel P
Abstract excerpt
A single G3:U70 base pair in the acceptor helix is the major determinant of alanine acceptance in alanine transfer RNAs. Transfer of this base pair into other transfer RNAs confers alanine acceptance. A G3:C70 substitution eliminates alanine acceptance in vivo and in vitro. In this work, a popula...
Topics
- Amino Acid Sequence
- Anticodon
- Base Composition
- Base Sequence
- Codon
- Escherichia coli
- Genes, Synthetic
- Kinetics
- Molecular Sequence Data
- Mutagenesis
- Nucleic Acid Conformation
- Phenotype
- RNA, Transfer, Ala
- Suppression, Genetic
- Tetrahydrofolate Dehydrogenase