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Article

Affinity maturation of tacrolimus antibody for improved immunoassay performance.

Clinical chemistry - 1 Jun 2008

Siegel Robert W, Baugher Wade, Rahn Tanya, Drengler Susan, Tyner Joan

Abstract excerpt

BACKGROUND: Organic solvents used for extraction of tacrolimus from whole blood samples lower the apparent affinity of the antibody used in a diagnostic immunoassay, thereby affecting the detection limit. METHODS: We used in vitro recombinant antibody engineering to screen and isolate clones from diverse libraries with mutagenic complementarity regions (CDRs) from tacrolimus 1-60-46 hybridoma cell line, with...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 18403566
DOI: 10.1373/clinchem.2007.097352

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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