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Article

Use of rpsL as a Counterselectable Marker in Borrelia burgdorferi.

Applied and environmental microbiology - 1 Feb 2010

Drecktrah Dan, Douglas J Miles, Samuels D Scott

Abstract excerpt

We have demonstrated that rpsL, encoding the S12 protein of the small ribosomal subunit, can be used as a counterselectable marker in Borrelia burgdorferi, the causative agent of Lyme disease. Mutations in rpsL confer streptomycin resistance. Streptomycin susceptibility is dominant in an rpsL merodiploid, and streptomycin selects for the loss of wild-type rpsL carried in trans. This is the first description of a...

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Identifiers

PMID: 19966024
DOI: 10.1128/AEM.02172-09
PMCID: PMC2813015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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