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Article

Use of rpsL for dominance selection and gene replacement in Streptomyces roseosporus

1 Jan 1997

Abstract excerpt

We developed a gene replacement system using the rpsL gene of Streptomyces roseosporus and demonstrated its utility by constructing a deletion in the S. roseosporus glnA gene. A 1.3-kb BamHI fragment that hybridized to the Mycobacterium smegmatis rpsL gene was subcloned from an S. roseosporus cosmid library and sequenced. Plasmid pRHB514 containing the rpsL gene conferred streptomycin sensitivity (Sm(S)) to the...

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Identifiers

PMID: 8981996
DOI: 10.1128/jb.179.1.180-186.1997

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Source receipt: pubmed, retrieved 7 Sept 2026.

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