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Article

Use of a dominant rpsL allele conferring streptomycin dependence for positive and negative selection in Thermus thermophilus.

Applied and environmental microbiology - 1 Aug 2007

Blas-Galindo Emilio, Cava Felipe, López-Viñas Eduardo, Mendieta Jesús, Berenguer José

Abstract excerpt

A spontaneous rpsL mutant of Thermus thermophilus was isolated in a search for new selection markers for this organism. This new allele, named rpsL1, encodes a K47R/K57E double mutant S12 ribosomal protein that confers a streptomycin-dependent (SD) phenotype to T. thermophilus. Models built on the available three-dimensional structures of the 30S ribosomal subunit revealed that the K47R mutation directly affects...

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Source

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Identifiers

PMID: 17601820
DOI: 10.1128/AEM.00751-07
PMCID: PMC1950985

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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