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Article

Efficient targeted mutagenesis in Borrelia burgdorferi.

Journal of bacteriology - 1 May 2000

Bono J L, Elias A F, Kupko J J, Stevenson B, Tilly K, Rosa P

Abstract excerpt

Genetic studies in Borrelia burgdorferi have been hindered by the lack of a nonborrelial selectable marker. Currently, the only selectable marker is gyrB(r), a mutated form of the chromosomal gyrB gene that encodes the B subunit of DNA gyrase and confers resistance to the antibiotic coumermycin A(1). The utility of the coumermycin-resistant gyrB(r) gene for targeted gene disruption is limited by a high frequency...

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PMID: 10762244
DOI: 10.1128/JB.182.9.2445-2452.2000
PMCID: PMC111306

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