Article
Hidden alternative structures of proline isomerase essential for catalysis.
Nature - 3 Dec 2009
Fraser James S, Clarkson Michael W, Degnan Sheena C, Erion Renske, Kern Dorothee, Alber Tom
Abstract excerpt
A long-standing challenge is to understand at the atomic level how protein dynamics contribute to enzyme catalysis. X-ray crystallography can provide snapshots of conformational substates sampled during enzymatic reactions, while NMR relaxation methods reveal the rates of interconversion between substates and the corresponding relative populations. However, these current methods cannot simultaneously reveal the...
Topics
- Catalysis
- Crystallography, X-Ray
- Cyclophilin A
- Humans
- Models, Molecular
- Mutation
- Protein Structure, Tertiary
- Temperature