Immortalized fibroblastoid cells of osteopetrotic mutant mice (op/op) do not secrete CSF-1 and do not inhibit CSF-1 activity released by normal cells.

Abstract excerpt

Osteopetrotic mutant mice (op/op) are deficient in osteoclasts and macrophages. In this report, the establishment of clonal permanent fibroblastoid cell lines from spleens of op/+ and op/op mice is described and conditioned medium obtained from these cell lines examined for release of growth factor that stimulates the proliferation and differentiation of macrophage precursor cells. It is shown that conditioned...

Topics

• Animals
• Cell Line
• Fibroblasts
• Gene Expression
• Hematopoietic Stem Cells
• Heterozygote
• Hybrid Cells
• Macrophage Colony-Stimulating Factor
• Mice
• Mice, Mutant Strains
• Mutation