Mutations in the Escherichia coli dnaG gene suggest coupling between DNA replication and chromosome partitioning.

doi:10.1128/jb.173.3.1268-1278.1991

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Article

Mutations in the Escherichia coli dnaG gene suggest coupling between DNA replication and chromosome partitioning.

Journal of bacteriology - 1 Feb 1991

Grompe M, Versalovic J, Koeuth T, Lupski J R

Abstract excerpt

Eleven conditional lethal dnaG(Ts) mutations were located by chemical cleavage of heteroduplexes formed between polymerase chain reaction-amplified DNAs from wild-type and mutant dnaG genes. This entailed end labeling one DNA strand of the heteroduplex, chemically modifying the strands with hydroxylamine or osmium tetroxide (OsO4) at the site of mismatch, and cleaving them with piperidine. The cleavage products...

Topics

Bacterial Proteins
Base Sequence
Chromosome Mapping
Chromosomes, Bacterial
DNA Primase
DNA Replication
DNA, Bacterial
Escherichia coli
Gene Expression Regulation, Bacterial
Genes, Bacterial
Hydroxylamines

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Microscopy, Fluorescence

Molecular Sequence Data

Mutation

Nucleic Acid Heteroduplexes

Osmium Tetroxide

Phenotype

Piperidines

Source

Open in PubMed Open full text

Identifiers

PMID: 1991720
DOI: 10.1128/jb.173.3.1268-1278.1991
PMCID: PMC207251

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1991720-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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