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Article

Mutations in the Escherichia coli dnaG gene suggest coupling between DNA replication and chromosome partitioning.

Journal of bacteriology - 1 Feb 1991

Grompe M, Versalovic J, Koeuth T, Lupski J R

Abstract excerpt

Eleven conditional lethal dnaG(Ts) mutations were located by chemical cleavage of heteroduplexes formed between polymerase chain reaction-amplified DNAs from wild-type and mutant dnaG genes. This entailed end labeling one DNA strand of the heteroduplex, chemically modifying the strands with hydroxylamine or osmium tetroxide (OsO4) at the site of mismatch, and cleaving them with piperidine. The cleavage products...

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Identifiers

PMID: 1991720
DOI: 10.1128/jb.173.3.1268-1278.1991
PMCID: PMC207251

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