Article
Rapid detection of K-ras mutations in bile by peptide nucleic acid-mediated PCR clamping and melting curve analysis: comparison with restriction fragment length polymorphism analysis.
Clinical chemistry - 1 Mar 2004
Chen Chiung-Yu, Shiesh Shu-Chu, Wu Sheu-Jen
Abstract excerpt
BACKGROUND: Current methods for detection of K-ras gene mutations are time-consuming. We aimed to develop a one-step PCR technique using fluorescent hybridization probes and competing peptide nucleic acid oligomers to detect K-ras mutations in bile and to compare the efficacy with restriction fra...
Topics
- Aged
- Bile
- Biomarkers, Tumor
- Cholangiocarcinoma
- Choledocholithiasis
- Codon
- Female
- Genes, ras
- Humans
- Lithiasis
- Male
- Middle Aged
- Mutation
- Pancreatic Neoplasms
- Peptide Nucleic Acids
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- ras Proteins