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Article

Rapid detection of K-, N-, H-RAS, and BRAF hotspot mutations in thyroid cancer using the multiplex primer extension.

Clinical biochemistry - 1 Oct 2013

Chang Ya-Sian, Lin I-Ling, Yeh Kun-Tu, Chang Jan-Gowth

Abstract excerpt

OBJECTIVES: The objective of this study is to develop a multiplex PCR and primer extension to detect K-, N-, H-RAS, and BRAF mutations. DESIGN AND METHODS: DNA samples were isolated from 76 thyroid cancer patients. Multiplex amplification of exons 2 and 3 of three RAS genes and exon 15 of the BRAF gene using three pairs of primers was performed in a single tube. The products were split into three tubes. First, we...

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Source

Identifiers

PMID: 23792105
DOI: 10.1016/j.clinbiochem.2013.06.005

Genes

BRAF

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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