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Article

Structural explanation for the acquisition of glycosynthase activity.

Journal of biochemistry - 1 Feb 2010

Hidaka Masafumi, Fushinobu Shinya, Honda Yuji, Wakagi Takayoshi, Shoun Hirofumi, Kitaoka Motomitsu

Abstract excerpt

Glycosynthases are engineered glycoside hydrolases (GHs) that catalyse the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors. We have determined five crystal structures of the glycosynthase mutants reducing-end xylose-releasing exo-oligoxylanase, an inverting GH, that exhibit various levels of glycosynthetic activities. At the active site of the Y198F mutant, the most efficient...

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Identifiers

PMID: 19819900
DOI: 10.1093/jb/mvp159

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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