Article
Transforming wheat vacuolar invertase into a high affinity sucrose:sucrose 1-fructosyltransferase.
The New phytologist - 1 Jan 2008
Schroeven Lindsey, Lammens Willem, Van Laere André, Van den Ende Wim
Abstract excerpt
Vacuolar invertases (VIs) degrade sucrose to glucose and fructose. Additionally, the fructan plant wheat (Triticum aestivum) contains different fructosyltransferases (FTs), which have evolved from VIs by developing the capacity to bind sucrose or fructans as acceptor substrates. Modelling studies revealed a hydrogen bonding network in the conserved WMNDPNG motif of VIs, which is absent in FTs. In this study, the...
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