Article
Involvement of the beta clamp in methyl-directed mismatch repair in vitro.
The Journal of biological chemistry - 20 Nov 2009
Pluciennik Anna, Burdett Vickers, Lukianova Olga, O'Donnell Mike, Modrich Paul
Abstract excerpt
We have examined function of the bacterial beta replication clamp in the different steps of methyl-directed DNA mismatch repair. The mismatch-, MutS-, and MutL-dependent activation of MutH is unaffected by the presence or orientation of loaded beta clamp on either 3' or 5' heteroduplexes. Similarly, beta is not required for 3' or 5' mismatch-provoked excision when scored in the presence of gamma complex or in the...
Topics
- Adenosine Triphosphatases
- Base Pair Mismatch
- Blotting, Western
- Catalytic Domain
- DNA Polymerase III
- DNA Repair
- DNA Repair Enzymes
- DNA, Superhelical
- DNA-Binding Proteins
- Endodeoxyribonucleases
- Escherichia coli