Article
Cse4 is part of an octameric nucleosome in budding yeast.
Molecular cell - 24 Sept 2009
Camahort Raymond, Shivaraju Manjunatha, Mattingly Mark, Li Bing, Nakanishi Shima, Zhu Dongxiao, Shilatifard Ali, Workman Jerry L, Gerton Jennifer L
Abstract excerpt
The budding yeast CenH3 histone variant Cse4 localizes to centromeric nucleosomes and is required for kinetochore assembly and chromosome segregation. The exact composition of centromeric Cse4-containing nucleosomes is a subject of debate. Using unbiased biochemical, cell-biological, and genetic approaches, we have tested the composition of Cse4-containing nucleosomes. Using micrococcal nuclease-treated...
Topics
- Centromere
- Chromatin Immunoprecipitation
- Chromosomal Proteins, Non-Histone
- DNA-Binding Proteins
- Gene Expression Regulation, Fungal
- Histones
- Models, Molecular
- Multiprotein Complexes
- Mutation
- Nucleic Acid Conformation
- Nucleosomes
- Protein Conformation
- Protein Multimerization