Article
Cloning of an aroF allele encoding a tyrosine-insensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase.
Journal of bacteriology - 1 Nov 1990
Weaver L M, Herrmann K M
Abstract excerpt
In Escherichia coli, genes aroF+, aroG+, and aroH+ encode isoenzymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthases that are feedback inhibited by tyrosine, phenylalanine, and tryptophan, respectively. A single base pair change in aroF causes a Pro-148-to-Leu-148 substitution and results in a tyrosine-insensitive enzyme.
Topics
- 3-Deoxy-7-Phosphoheptulonate Synthase
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Genes, Bacterial
- Isoenzymes
- Molecular Sequence Data
- Plasmids
- Restriction Mapping
- Tyrosine