Article
Identification of the promoter, operator, and 5' and 3' ends of the mRNA of the Escherichia coli K-12 gene aroG.
Journal of bacteriology - 1 May 1990
Baseggio N, Davies W D, Davidson B E
Abstract excerpt
The promoter, operator, and 5' and 3' ends of the mRNA of the Escherichia coli gene aroG (encoding the phenylalanine-sensitive 3-deoxy-arabinoheptulosonate-7-phosphate synthase) were located. Primer extension analysis and nuclease S1 mapping of in vivo transcripts were used to determine the 5' and 3' ends, respectively, of the mRNA. Both ends exhibited some heterogeneity with respect to length. The 3' end of the...
Topics
- 3-Deoxy-7-Phosphoheptulonate Synthase
- Aldehyde-Lyases
- Base Sequence
- DNA, Recombinant
- Deoxyribonuclease I
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligonucleotide Probes