Article
Phosphate-affinity polyacrylamide gel electrophoresis for SNP genotyping.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Kinoshita Eiji, Kinoshita-Kikuta Emiko, Koike Tohru
Abstract excerpt
We introduce a genotyping method which relies on the use of a 1:1 mixture of 5'-phosphate-labeled and nonlabeled allele-specific primers for polymerase chain reaction (PCR). The method is based on the difference in mobility of the phosphorylated and nonphosphorylated PCR products (possessing the same number of base pairs) during phosphate-affinity polyacrylamide gel electrophoresis (PAGE). The phosphate-affinity...
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