Article
Sequencing using capillary electrophoresis of short tandem repeat alleles separated and purified by high performance liquid chromatography.
Electrophoresis - 1 Jan 1998
Marino M A, Devaney J M, Smith J K, Girard J E
Abstract excerpt
Polymerase chain reaction (PCR) amplified alleles need to be isolated and purified before carrying out additional analysis to confirm sequence, number of repeats and microvariants within a short tandem repeat (STR) locus. Also, PCR amplification of tetranucleotide repeat loci, used in DNA typing assays, often result in heteroduplex formation, adding to the complexity of analysis. Sequencing reactions require...
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